tnt buffer (Thermo Fisher)

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Thermo Fisher tnt buffer
Tnt Buffer, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/tnt+buffer/Tween+20/pmc12848019-567-6-34
Average 99 stars, based on 1 article reviews

tnt buffer - by Bioz Stars, 2026-10

99/100 stars

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Incubation:

Article Title: Chemotherapy-induced adipo-lineage cell senescence drives bone loss.
Article Snippet: .. Next day, AR TI CL E IN P RE SS sections were washed with TNT buffer (0.1 M Tris–HCL pH 7.4, 0.15 M sodium chloride, 0.1% Tween-20) followed by incubation with secondary goat anti–rabbit Alexa Fluor 568 antibody (1:400, Cat. No. A-11011, Invitrogen) and goat anti–mouse Alexa Fluor 488 antibody (1:500, Cat. No. A-11001, Invitrogen). .. All sections were stained and mounted using SlowFade Gold antifade reagent with DAPI (Invitrogen S36939) according to manufacturer instruction.

Article Title: Fate plasticity of interneuron specification
Article Snippet: Antigen retrieval was performed using a solution containing 10 mM trisodium citrate dihydrate (Millipore Sigma # S1804) and 0.05% Tween 20 (Millipore Sigma # P1379) at pH 6.0, heated to 95°C for 10 minutes. .. Samples were washed with TNT buffer (100 mM Tris-HCl [Thermo Fisher Scientific # 15567027], 150 mM sodium chloride [Millipore Sigma # S9888], and 0.1% Tween 20) for 10 minutes, repeated three times, then incubated with 1% hydrogen peroxide (Millipore Sigma # H1009) in PBS for 2 hours. .. Slides were blocked for 2 hours with TNB solution (100 mM Tris-HCl, 150 mM sodium chloride, and 0.36% w/v bovine serum albumin [BSA] [Millipore Sigma # A2153]).

Article Title: Chemotherapy-induced adipo-lineage cell senescence drives bone loss
Article Snippet: .. Next day, sections were washed with TNT buffer (0.1 M Tris–HCL pH 7.4, 0.15 M sodium chloride, 0.1% Tween-20) followed by incubation with secondary goat anti–rabbit Alexa Fluor 568 antibody (1:400, Cat. No. A-11011, Invitrogen) and goat anti–mouse Alexa Fluor 488 antibody (1:500, Cat. No. A-11001, Invitrogen). .. All sections were stained and mounted using SlowFade Gold antifade reagent with DAPI (Invitrogen S36939) according to the manufacturer's instructions.

other:

Article Title: The Kinase CK1α coordinates Initiation and Termination of the cGAS-STING Pathway
Article Snippet: Single plane images were acquired by confocal microscopy (Nikon A1rHD LFOV) with a 60x oil-immersion lens at the MicroPICell core facility (SFR Bonamy, BioCore, Inserm UMS 016, CNRS UAR 3556, Nantes, France).

Membrane:

Article Title: Trpv6 channel targeting using monoclonal antibody induces prostate cancer cell apoptosis and tumor regression
Article Snippet: Total protein samples were subjected to 8% SDS-PAGE and transferred to a PVDF membrane by semi-dry Western blotting (Bio-Rad Laboratories). .. The membrane was blocked in fat-free 5% milk containing TNT buffer (Tris-HCl, pH 7.5, 140 mM NaCl, and 0.05% Tween 20) for 1 h, washed, and then probed overnight using mab82 anti-TRPV6 antibody (2.4 ng/μL), mouse monoclonal anti-β-actin (Lab Vision Co., 1/1000), mouse monoclonal anti-calpain 2 (ABclonal, A0900; 1/1000), mouse monoclonal anti-GAPDH (Abcam, AB8245, 1/1000), antibodies, mouse monoclonal anti-cytochrome c (Santa-cruz, sc13156, 1/1000), mouse monoclonal anti-caspase 9 (Santa-cruz, sc56076, 1/1000), mouse monoclonal anti-bax (Santa-cruz, sc23959, 1/1000), antibodies. .. Goat polyclonal anti-rabbit and anti-mouse peroxidase-conjugated secondary antibodies (Chemicon International; 1/200) were used.

Fluorescence:

Article Title: Unique spatially and temporary-regulated/sex-specific expression of a long ncRNA, Nb-1 , suggesting its pleiotropic functions associated with honey bee lifecycle
Article Snippet: Biotin-labeled riboprobes were detected using TSA Plus Fluorescein System (PerkinElmer) according to the manufacturer’s instructions. .. After counterstaining with 30 mM 4′-6-diamidino-2-phenylindole (DAPI, Invitrogen) in TNT buffer (100 mM Tris–HCl pH 7.5; 150 mM NaCl; 0.05% Tween 20), the signals were examined under the fluorescence microscope (Carl Zeiss) or the confocal laser-scanning microscope (LSM 710, Carl Zeiss). ..

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